Pre-early functions of bacteriophage T5 and its relatives
نویسندگان
چکیده
منابع مشابه
Cell-free transcription and translation of isolated restriction fragments localize bacteriophage T5 pre-early genes.
In vitro transcription and translation of isolated restriction fragments containing all or part of the terminal redundancies of bacteriophage T5 localized virtually every pre-early gene to a small 6.3-kilobase BglI fragment. Among these genes were those encoding the A1 and A2 proteins, which are responsible for complete entry of the viral genome into its host, and the deoxynucleoside 5'-monopho...
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Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.
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Nonsense mutations in structural genes appear to abort polypeptide chain synthesis at the amino acid site governed by the mutant codon. The two presently recognized types of nonsense mutant, amber and ochre, have been assigned the codons UAG and UAA, respectively. Certain bacteria harbor one or more genetic suppressors that counteract the effect of appropriate nonsense mutations in their own st...
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A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototy...
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Infection of a susceptible bacterium by a virulent phage particle is followed, after a latent period, by cell lysis and liberation of newly formed phage particles. The infecting particle loses its infectivity shortly after adsorption to the host, a fact attributed to disociation of the particle into a-cell penetrating fraction, consting chiefly of nucleic acid, and an externally persisting frac...
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ژورنال
عنوان ژورنال: Bacteriophage
سال: 2015
ISSN: 2159-7081
DOI: 10.1080/21597081.2015.1086500